A study, published in Archives of Oral Biology, sought to understand how CBD, CBN, and the two of them combined, in the form of local intramuscular injections could decrease masticatory muscle sensitization, caused by nerve growth factor.

From a behavioral standpoint, both CBD (5mg/ml) and CBN (1mg/ml) decreased mechanical sensitization, and when combined in 1:1 ration, the effects lasted for longer.

In line with the behavioral results, both compounds alone, as well as the two of them combined, “increased the mechanical threshold of masseter muscle mechanoreceptors.” However, quite interestingly, when significantly increasing the amount of CBD in the combination, and respectively the ratio — CBD/CBN (5:1 mg/ml) — actually had a reverse effect, decreasing the analgesic effect.

Other studies have stumbled upon this paradoxical property of CBD — the more the better isn’t necessarily the case with it, as too much of it can actually hinder its effects. It seems as this phenomenon is apparent, if not even more pronounced, when CBD is combined with CBN. At the same time, CBD and CBD seem to synergize in the right ratio and quantities, which is also in line with other studies, as well as cannabinoids’ famous “entourage effect” as a whole.

Overall, CBD and CBN certainly demonstrated potential for alleviating muscle pain.

“These results suggest that peripheral application of these non-psychoactive cannabinoids may provide analgesic relief for chronic muscle pain disorders such as temporomandibular disorders and fibromyalgia without central side effects,” the study’s authors state.